Leica TCS NT Microscope

Confocal microscopy allows the user to obtain depth-selective information on the three dimensional structure of a microscopic object. The confocal principle is especially valuable in fluorescence microscopy, since it almost completely eliminates stray light not coming from the focal plane. Thus the system is able to produce fluorescence images with optimum clarity and resolution of fine details.

Configuration

  • Four laser excitation (Ar-UV, Ar-488, Kr-568, HeNe-633)
  • Four fluorescent detectors and transmitted detector
  • Objectives:
    • 100x Plan APO 1.4 NA oil immersion
    • 63x Plan APO 1.2 NA water immersion
    • 63x HCX APO 0.9 NA dipping
    • 40x HCX APO 0.8 NA dipping
    • 40x Plan Fluotar 1.0 NA oil immersion
    • 10x Plan Fluotar 0.3 NA dry
  • Images analyzed using the Leica TCS and Volocity software

Calendar

Online Scheduling for trained users only. Contact Jim Henthorn for assistance.
A schedule calendar for the Leica TCS is available online at http://flow-server/confocal

Pricing

With operator assistance $40/hour
Without operator assistance (must be trained) $20/hour
Cancellation Charges (less than 24-hour notice)  $35

Links