Leica SP2 MP Confocal

The highly efficient SP prism spectrophotometer for detection of fluorescence emission coupled with the AOBS (acoustical optical beam splitter) allows users to collect more signal than using standard optical filters. With the use of the multi-photon laser the microscope system becomes inherently confocal also allowing deeper penetration into tissue with less photo-bleaching.

Configuration

  • Six laser excitation plus multi-photon which produce the following excitation: 405, 488, 458, 476, 488, 496, 514, 514, 543, 594, 633, 710-980nm (from the MP laser)
  • Two fluorescent detectors and transmitted detector
  • Objectives:
    • 100x Plan APO 1.4 NA oil immersion
    • 63x Plan APO 1.4 NA oil immersion
    • 63x HCX APO 0.9 NA dipping
    • 40x HCX APO 1.25 NA oil immersion
    • 40x HCX APO 0.8 NA dipping
    • 10x HC PL Fluotar 0.3 NA dry
  • Images analyzed using the Leica TCS and Volocity software

Calendar

Online Scheduling for trained users only. Contact Jim Henthorn for assistance.
A schedule calendar for the Leica SP2 is available online at http://flow-server/SP2 for trained users.

Pricing

With operator assistance $50/hour
Without operator assistance (must be trained) $30/hour
Cancellation Charges (less than 24-hour notice)  $35

Links