The ISS Chronos Lifetime Spectrometer

The Chronos is the first frequency-domain, fluorescence lifetime instrument based on the use of laser diodes and light emitting diodes (LED) as the sources for excitation. The light source modules are easily interchangeable when a user requires operation at other excitation wavelengths. The instrument is fully automated: the rotating cuvette holder, the polarizers, the three shutters, the monochromators, and the gain of the photomultiplier tubes are all computer-controlled. A variety of experimental protocols allows for a variety of measurements including fluorescence intensity and anisotropy decay, quenching and resonance energy transfer.

 The Vinci Multidimensional Fluorescence Spectroscopy Analysis software (Windows ME/XP) controls the instrument automation and its interface to computer-controlled external devices. Vinci includes routines for the automatic acquisition of unidimensional and multidimensional data files The analysis portion of the software includes data manipulation (operations between spectra, smoothing, correction, derivative and integration). The graphical display allows for 2D and 3D plots as well color display of user defined functions with zooming and rotation capabilities, statistical operators, and plot export to popular formats (metafile, bitmap).

For further information contact:

Jialing Lin, Ph.D. (faculty webpage)

Assistant Professor

Department of Biochemistry and Molecular Biology

Tel: (405) 271-2227 ext.61216

email: jialing-lin@ouhsc.edu